Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00184
OS Name
Optogenetic regulate iTrkA activity by iLID system (Opto-iTrkA-iLID)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Improved Light-Inducible Dimer (iLID)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
747155673
PDB ID
4WF0
3D Structure
Components
Photoreceptor: Improved Light-Inducible Dimer (iLID)
Complementary Protein: Stringent starvation protein B (SspB)
Cofactor: Flavin mononucleotide (FMN)
Complementary Protein Stringent starvation protein B (SspB)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Recruited protein Stringent starvation protein B
Detail Info
Fusion protein Tropomyosin receptor kinases A
Detail Info
OS Application Information (1)
Mechanism of Action ILID comformational change and subsequently iLID-SspB heterodimerization [ 1]
Controlled Signal Pathway TrkA signal activation
Effect Activation
Application Light-dependent protein translocation to activate iTrkA by iLID
Note Tdnano is a tandem dimer of iLID binding partner SspB
Light Information Activation Wavelength 488 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Minutes
Light Intensity 9 watts per square centimeter; 400 microwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines PC12 cells
References
1 Construction of Light-Activated Neurotrophin Receptors Using the Improved Light-Induced Dimerizer (iLID)