Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00183
|
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| OS Name |
Light regulated signal protein localization and activity system 2
(LRPLA 2)
|
[1] | ||||
|
Photoreceptor (PR) |
|
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| Complementary Protein | Stringent starvation protein B (SspB) | |||||
| Cofactor | Flavin mononucleotide (FMN) | |||||
| Controlled Protein | Functional protein | Improved Light-Inducible Dimer | ||||
| Recruited protein | Stringent starvation protein B | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | ILID comformational change and subsequently iLID-SspB heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Protein translocation | |||||
| Effect | Sensor | |||||
| Application | Light mediated subcellular localization in mammalian cell culture and reversible control of small GTPase signaling | |||||
| Note | Switches from 130 nM under blue light to 4.7 M in the dark | |||||
| Light Information | Activation Wavelength | 488 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | Seconds | |||||
| Deactivation Time | Minutes | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | Mouse fibroblasts | |||||