Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00174
OS Name
Optogenetic JNK inhibit system 2 (OptoJNKi 2)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (C450A) (AsLOV2 (C450A))
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
Mutation Site
C450A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (C450A) (AsLOV2 (C450A))
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein Minimal JNK-binding protein 1
Detail Info
OS Application Information (1)
Mechanism of Action Comformational change [ 1]
Controlled Signal Pathway JNK inhibition (permanent dark contrast)
Application A permanent dark contrast for study the OptoJNKi system
Note Used for comparative study
Light Information Activation Wavelength .
Deactivation Wavelength Permanent in dark state
Expression Information Expression Method Transfection
Cell Lines HEK 293T cells
References
1 A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs