Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00170
OS Name
Blue light-inducible degradation domain (B-LID)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein Degradation peptide
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Controlled Signal Pathway Protein degradation
Effect Inhibition
Application Optogenetic system to down-regulate protein levels by proteasome-mediated degradation
Note Dual-control protein levels with blue-off
Light Information Activation Wavelength 460 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time 80 s
Light Intensity 20 micromoles per square meter per second
Expression Information Expression Method Transfection
Cell Lines HEK-293T; HeLa cells; COS-7 cells and CHO-K1 cells and NIH 3T3 cells
References
1 Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells