Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00169
OS Name
Optogenetic modulation of REST target gene system 2 (Opto-REST 2)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein First N-terminal of REST interacting LIM domain protein
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Controlled Signal Pathway Competes with REST-DNA by binding to Zn-finger domains of the repressor factor
Effect Inhibition
Application Regulation of neural gene transcription by optogenetic inhibition of the RE1-silencing transcription factor
Note RILP strategy control transcription factor
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 12 h
Deactivation Time 12 h
Light Intensity 0.34 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines Murine neuro2a neuroblastoma cells
References
1 Regulation of neural gene transcription by optogenetic inhibition of the RE1-silencing transcription factor