Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00166
OS Name
Optogenetic control protein-protein interaction system 3 (Opto-PPI 3)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein Binder of cPYP-light state
Detail Info
OS Application Information (1)
Mechanism of Action BoLD-AsLOV2 dissociation [ 1]
Controlled Signal Pathway Protein translocation
Effect Sensor
Application Phage display to discover selective binder for photoswitchable proteins for protein localization
Note BoLD bind to dark state AsLOV2 only
Light Information Activation Wavelength 450 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time <2 s
Deactivation Time 3 min
Expression Information Expression Method Transfection and Transformation
Cell Lines E. coli K12
References
1 Discovering Selective Binders for Photoswitchable Proteins Using Phage Display