Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00165
OS Name
Bue light-inducible nuclear export system (LEXY)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Functional protein LOV2 domain of Avena sativa Phototropin 1
Detail Info
Fusion protein Nuclear export sequence
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Controlled Signal Pathway Nucleocytoplasmic protein transport
Effect Activation
Application Optogenetic control of nucleocytoplasmic protein export
Note LEXY=AsLOV2+NES
Light Information Activation Wavelength 440-490 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Within 1 min
Expression Information Expression Method Transfection
Cell Lines HEK293T cells
References
1 Optogenetic Control of Nucleocytoplasmic Protein Transport