Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00160
OS Name
Optogenetic p38MAPK inhibit system 1 (Optop38i 1)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein Putative p38MAPK inhibitor
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Controlled Signal Pathway p38MAPK inhibition
Effect Inhibition
Application Light-envoked inhibition of MLK-envoked p38 activation
Note A larger lsm/dsm pulldown ratio than earlier variants
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Tens of seconds
Light Intensity 6.4 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines HEK 293T cells
References
1 A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs