Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00159
OS Name
Optogenetic JNK inhibit system 1 (OptoJNKi 1)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein Minimal JNK-binding protein 1
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Controlled Signal Pathway JNK inhibition
Effect Inhibition
Application A light-regulated JNK inhibitor to inhibit endogenous c-Jun phosphorylation for genetranfer or protein delivery
Note Temperature dependent recovery rate
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Tens of seconds
Light Intensity 6.4 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines HEK 293T cells
References
1 A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs