Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00158
OS Name
Optogentic HA4 (OptoHA4)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein A monobody
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Controlled Signal Pathway Monobody binds to respective binder
Effect Activation
Application Light responsive protein binding to monobody to regulate tyrosine-protein kinase ABL1 activity
Note Possibly to make monobody to interact with specific interaction light dependent
Light Information Activation Wavelength 447 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 2 min
Deactivation Time Minutes
Light Intensity 45 micromoles per square meter
Expression Information Expression Method Transfection
Cell Lines HEK293T cells
References
1 Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold