Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00154
OS Name
Optogenetic regulate SOAR function system (OptoSOAR)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein STIM-Orai activating region
Detail Info
OS Application Information (1)
Mechanism of Action Allosteric Comformational changes [ 1]
Controlled Signal Pathway STIM1-gated Ca2+ influxion
Effect Activation
Application Uncaging the SOAR-contailng STIM1ct fragments to triger Ca2+ influx
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 7.2 ± 1.2 s
Deactivation Time 28.7 ± 4.6 s
Light Intensity 4.0 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines Hela cells
References
1 Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling