Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00152
OS Name
Blue-lightCinduced K+ channel 1 (BLINK 1)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein Potassium channel protein kcv
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Controlled Signal Pathway Neuron silence
Effect Inhibition
Application Establish prolonged physiological hyperpolarization of cells at low light intensities by a single component optogenetic tool
Note BLINK1 exhibits biophysical features of Kcv including K+ selectivity and high single-channel conductance
Channel Type Potassium channel
Light Information Activation Wavelength 455 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 7.6 s
Deactivation Time 38 s
Light Intensity 40 microwatts per square millimeter
Expression Information Expression Method Transfection
Cell Lines HEK293 cells and Zebrafish larvae
References
1 Optogenetics. Engineering of a light-gated potassium channel