Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00075
OS Name
Photoactivatable tetracycline controlled gene expression system (PA-Tet-ON/OFF)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Residue 1-531 of cryptochromes 2 (L348F) (CRY2 (1-531) (L348F))
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6K8I
Mutation Site
L348F
3D Structure
Components
Photoreceptor: Residue 1-531 of cryptochromes 2 (L348F) (CRY2 (1-531) (L348F))
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Recruited protein Tetracycline repressor protein class D
Detail Info
Fusion protein Transcription factor p65
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIB1 heterodimerization [ 1]
Controlled Signal Pathway Gene expression activation
Effect Activation
Application Tet gene expression system control by light and dox
Note PA-Tet-OFF activate downstream signals in the absense of Dox while PA-Tet-ON active downstream signals in the presence of Dox
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 1-2 min
Deactivation Time 3 h
Light Intensity 1.7 watts per square meter
Expression Information Expression Method Transfection
Cell Lines Mouse hippocampal neurons
References
1 Light Control of the Tet Gene Expression System in Mammalian Cells