Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00074
OS Name
Second generation of light-activated BCL2-associated X apoptosis regulator (OptoBax 2.0)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Residue 1-531 of cryptochromes 2 (L348F) (CRY2 (1-531) (L348F))
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6K8I
Mutation Site
L348F
3D Structure
Components
Photoreceptor: Residue 1-531 of cryptochromes 2 (L348F) (CRY2 (1-531) (L348F))
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein BCL2-associated X apoptosis regulator
Detail Info
Recruited protein Mitochondrial import receptor subunit TOM20
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIB1 heterodimerization [ 1]
Controlled Signal Pathway BAX activation
Effect Activation
Application Light initiated apoptosis with less frequent light stimulation
Light Information Activation Wavelength 470 nm
Process Ireversible
Activation Time Minutes
Expression Information Expression Method Transfection
Cell Lines Neuro-2a and HEK293T cells
References
1 Imaging of morphological and biochemical hallmarks of apoptosis with optimized optogenetic tools