Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00072
OS Name
Optogenetic Controlled G-protein signaling system (Dark state) (Opto-RGS2(D387A))
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (D387A) (CRY2PHR (D387A))
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
Mutation Site
D387A
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (D387A) (CRY2PHR (D387A))
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Human G-protein signaling 2
Detail Info
Recruited protein Regulator of G-protein signaling 2
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIBN heterodimerization [ 1]
Application Termination of Galphaq signaling and downstream release of calcium from the endoplasmic reticulum (dark mutation)
Note Dark mutant
Light Information Activation Wavelength Dark mutant
Process Ireversible
Activation Time Dark mutant
Expression Information Expression Method Transfection
Cell Lines HEK293T cells
References
1 Optogenetic Inhibition of G(q) Protein Signaling Reduces Calcium Oscillation Stochasticity